Peroxiredoxin II, His-tag Recombinant

Catalog #

70011

$550 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Peroxiredoxin 2, also known as PRDX2, full length (Genbank accession number NM_005809) with N-terminal His-tag, MW=22.7 kDa, expressed in Baculovirus infected Sf9 cell expression system.

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Synonyms

PRDX2

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Product Data Gallery

14% SDS-PAGE Coomassie Staining

PRDX2 Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1.35 mM KCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

22.7 kDa

Amino Acids

full length

Specific Activity

≥149 pmol/min/µg

Genbank #

NM_005809

UniProt #

P32119

Tag(s)

N-terminal His-tag

References

1. Peskin,A.V., et al., J. Biol. Chem. 282 (16), 11885-11892 (2007)
2. Low,F.M., et al., Blood 109 (6), 2611-2617 (2007)

Storage/Stability

>6 months at –80°C.

Assay Conditions

3.45 µg of PRDX2 was incubated in 50 mM HEPES (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30°C for 5 min, followed by the addition of 0.22 mM H₂O₂. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of biochemical function, enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Peroxiredoxin

Regulatory Status

Research Use Only

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Datasheets/Protocols

70011_Lot2003.pdf

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Peroxiredoxin II, His-tag Recombinant

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