Human PEDF Recombinant

Catalog #

90229-A

$130 *

Size: 2 µg

Qty

Bulk/Custom

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Description

Recombinant human pigment epithelium-derived factor (PEDF) is a disulfide-linked monomer protein consisting of 440 amino acid residue subunits, and migrates as an approximately 45k Da protein under non-reducing and reducing conditions in SDS-PAGE. Source: Optimized DNA sequence encoding human PEDF mature chain was expressed in E. coli.

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Synonyms

Pigment epithelium-derived factor, PEDF, Cell Proliferation-Inducing Gene 35 Protein, EPC-1, Serpin F1, SERPINF1, EPC-1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered 20 mM phosphate buffer, 150 mM NaCl solution, pH 7.5.

45 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

20–418

Genbank #

P36955

UniProt #

P36955

Background

Pigment epithelium-derived factor (PEDF), a 50- kDa secreted glycoprotein, is widely expressed throughout the human body, with expression decreasing during human hepatocellular carcinoma and breast cancer progression. PEDF binds to a cell membrane receptor to exhibit multifunctional activity in many cell types but its signaling mechanisms are largely unknown. PEDF exerts anti-angiogenic activity by arresting VEGF- or bFGF-mediated endothelial cell migration, inhibiting capillary morphogenesis, and inducing endothelial cell apoptosis. In human dermal microvascular cells, PEDF induces FasL expression and subsequently activation of caspase-8, which initiates the downstream apoptotic cascade. In human umbilical vein endothelial cells (HUVECs), its induction of apoptosis was shown to depend on p38 MAPK activity and to involve activation of caspases-8 and -9.

References

1. Craword SE, et al. Expert Opin Drug Discov. 2013 Jul,8(7):769-92.
2. Alcantara MB, Dass CR. Cell Physiol Biochem. 2013,31(4-5):487-94.
3. Becerra SP, Notario V. Nat Rev Cancer. 2013 Apr,13(4):258-71.

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Human PEDF Recombinant

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