Human Platelet Derived Growth Factor-AA Recombinant

Catalog #

90228-A

$130 *

Size: 2 µg

Qty

Bulk/Custom

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Description

Recombinant PDGF-AA is a disulfide-linked homodimeric protein consisting of two 126 amino acid residues, migrates as an approximately 28 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human PDGF-AA mature chain was expressed in E. coli.

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Synonyms

PDGFAA, Platelet-Derived Growth Factor Alpha Chain, PDGF-AA

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥98% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.

14 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

87–211

Biological Activity

The ED₅₀ was determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/ml, corresponding to a specific activity of > 1 x 10⁶ units/mg

Genbank #

P04085

UniProt #

P01127

Background

PDGF is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets. Platelets synthesize a mixture of the three possible isoforms (BB, AB, AA) while fibroblasts stimulated with EGF synthesize AA homodimers. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells, and endothelial cells. Two related receptors, are PDGFRalpha (CD140a) or PDGFRbeta ( CD140b). In contrast to many other cytokines PDGF is not released into the circulation. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix which facilitates local concentration of the factor. The factor functions as a local autocrine and paracrine growth factor. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF regulates the synthesis of its own receptor and also influences the expression of membrane receptors for IL-1, EGF, 5-Hydroxytryptamine, LDL, transferrin, and muscarinergic receptor.

References

1. Am J Physiol Renal Physiol, Feb 2009, 296: F406 - F417.
2. Nephrol. Dial. Transplant., Apr 2005, 20: 790 - 796.
3. J. Cell Sci., Mar 1999, 112: 905 - 915.

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Human Platelet Derived Growth Factor-AA Recombinant

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