PDE4C1, GST-tag Recombinant

Catalog #

60044

$470 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Human PDE4C1 (GenBank Accession No. NM_000923), full length, with N-terminal GST-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 8 times.

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Synonyms

PDE4C, phosphodiesterase 4C, PDE4-C1, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations8

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥41%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

106 kDa

Amino Acids

full length

Specific Activity

≥3,000 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_000923

UniProt #

Q08493

Tag(s)

N-terminal GST-tag

References

Wang H. et al., Biochem J. 2007; 408(2):193-201.
2. Sullivan M., Olsen A.S. and Houslay M.D. Cell. Signal. 1999; 11 (10):735-742.

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 1 mM MnCl₂, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4C1 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only