Normal Human Peripheral Blood Mononuclear Cells, Frozen
Cryopreserved vial (100 x 106, 30 x 106, 10 x 106 or 2 x 106 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis /apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.
Materials Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 10 | BPS Bioscience #79704 |
Human peripheral blood mononuclear cells (PBMCs) include all the cells present in the peripheral blood that have a round nucleus. It is thus a mix of lymphocytes, such as T, B and NK cells, and monocytes. Most cells present in PBMCs are lymphocytes, with T cells being the main cell type. The proportion of each cell type present can be determined by using cell specific marker combinations, for instance NK cells are characterized as CD3-CD56+ cells. The use of these markers also allows for isolation of cell populations of interest. PBMCs are a critical and widely used cell source in the immunology, infectious disease, vaccine, cancer therapy and transplant immunology fields.
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