Human Noggin, also known as symphalangism 1 (SYM1) or synostoses (multiple) syndrome 1 (SYNS1), GenBank Accession No. NM_005450, a.a. 28-232(end), disulfide-linked homodimer. MW=~28kDa (monomer). Source: human 293 cells. Endotoxin Level: <1 EU/μg. Biological Activity: measured by Noggin’s ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is < 20 ng/ml in the presence of 30 ng/ml of human BMP-4. Formulation: Lyophilized from a 0.2 μM filtered, aqueous solution. Reconstitution: Supplied as a lyophilized solid. Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA.
Lyophilized from a 0.2 µm filtered 0.1 mg/ml solution of 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA
MW
28 kDa
Endotoxin Level
< 1EU per µg of Noggin as determined by the LAL method.
Amino Acids
28–232(end)
Biological Activity
Measured by Noggin’s ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is <20 ng/ml in the presence of 30 ng/ml of human BMP-4.
Genbank #
NM_005450
UniProt #
Q13253
Tag(s)
none
Background
Noggin is one of a group of proteins that act as secreted antagonists of BMP activity. BMPs are essential for osteogenesis and organogenesis during embryonic development, and also play a role in tissue healing in adults. Noggin inhibits BMP activity by binding to them with high affinity, blocking their ability to bind to their receptor.
References
1. Tao, Y.X., et al. (2010). Indian J. Exp. Biol. 48(5):444-52. 2. Mfopou, J.K., et al. (2010). Gastroenterology. 138(7):2233- 2245. 3. Chaturvedi, G., et al. (2009). Cell Prolif. 42(4):425-33
Storage/Stability
≥12 months (lyophilized) at -20°C. Store reconstituted protein in aliquots at -20°C to –70°C for up to 6 months. Avoid freeze/thaw cycles.
Reconstitution
Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Applications
Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
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