Human beta Nerve Growth Factor Recombinant

Catalog #

90223-A

$130 *

Size: 5 µg

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Description

Recombinant human NGF is as a non disulfide-linked homodimeric protein consisting of two 118 amino acid residues, and migrates as a 13 - 14 kDa protein under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Nerve Growth Factor mature chain was expressed in Chinese Hamster Ovary (CHO) cells.

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Synonyms

NGFbeta, B-NGF, Beta-Nerve Growth Factor

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Hamster

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered 20 mM sodium acetate, 150 mM NaCl, pH 5.5.

13 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

122–241

Biological Activity

The ED₅₀ was determined by the determined by its ability to proliferate TF-1 cells and was found to be in the range of 0.5 ng/ml.

Genbank #

P01138

UniProt #

P01138

Background

NGF is mainly responsible for the survival and the differentiation and the functional activities of sensory and sympathetic neurons in the peripheral nervous system. It also plays an important role in the development and functional activities of cholinergic neurons in the central nervous system. Since NGF is synthesized also in non-neuronal tissues it may have a much wider spectrum of biological activities than thought previously. NGF stimulates chemotactic migration of human polymorphonuclear leukocytes in vitro. NGF stimulates the growth and differentiation of B-cells and the growth of T-cells and of some tumor cell types. NGF inhibits immunoglobulin production by various human plasma cell. The cytokines IL-1, IL-6, and bFGF are potent inducers of NGF. NGF induces the synthesis of IL-1 in pheochromocytoma cells which in turn acts as a growth factor for glial cells and induces the synthesis of NGF following nerve injuries. In thymic stromal cells NGF induces the synthesis of IL-6. NGF induces the synthesis of the fos oncogene and the myc oncogene and also influences the expression of EGF. One receptor that is responsible for mediating most of the activities of NGF is expressed preferentially in neuronal tissues. This glycoprotein of 140 kDa is the product of the trk gene. It possesses an intrinsic tyrosine-specific protein kinase in its intracellular domain.

References

1. Chaves RN, et al. Zygote. 2013 May,21(2):187-97.
2. Cuello AC. Neurochem Res. 2012 Jun,37(6):1256-60.
3. Berry A, et al. Neural Plast. 2012,2012:784040.

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Human beta Nerve Growth Factor Recombinant

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