MMP3(K45E), His-tag Recombinant

Catalog #

11346

$425 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human matrix metallopeptidase-3 (MMP3), also known as Stromelysin-1 (STMY1), GenBank Accession No. NM_002422, a.a. 18-477(end) with a Lys to Glu mutation at a.a. 45 and a C-terminal His-tag, expressed in a HEK293 cell expression system. MW=54 kDa.

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Synonyms

Stromelysin-1, Stromelysin, STMY, MMP-3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

MMP3 Activity

MMP3 (K45E) inhibition curve using a fluorogenic assay (#79907). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole, and 20% glycerol.

54 kDa

Amino Acids

18-477(end)

Genbank #

NM_002422

UniProt #

P08254

Tag(s)

C-terminal His-tag

References

1. Sternlicht, M.D., et al., Cell, 1999; 98(2):137-146.
2. Buttice, G., et al., Oncogene. 1996; 13(11):2297-2306.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay buffer: 25 mM Tris pH 9.0, 25 μM ZnCl₂, 0.05% Tween-20 Enzyme was diluted to 100 ng/μl in a solution containing 1 mM AMPA (amino-phenyl mercuric acetate) in assay buffer activated for 1 hour at 37°C. Then, 25 μl of 2X 2-fold serial dilution enzyme titration in assay buffer was mixed with 25 μl of 20 μM substrate solution (Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate, R&D Systems, ES002, 10 mg/ml = 6 mM stock in DMSO, 10 μM final assay concentration) in a Corning 384-well HV black microplate. Fluorescence was kinetically recorded at 320 nm excitation/405 nm emission for 30 minutes.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Protease

Regulatory Status

Research Use Only