Human Macrophage Inflammatory Protein-1 beta Recombinant

Catalog #

90218-A

$130 *

Size: 2 µg

Qty

Bulk/Custom

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Description

Recombinant MIP-1 beta is a disulfide-linked homodimeric protein consisting of 70 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human MIP-1 beta chain was expressed in E. coli.

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Synonyms

CCL4, Chemokine (C-C Motif) Ligand 4, Macrophage Inflammatory Protein 1-Beta, MIP-1-Beta, MIP1B, SCYA4, LAG1, ACT2

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution.

8 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

24–92

Biological Activity

Determined by its ability to chemoattract human blood monocytes using a concentration range of 2.0-10.0 ng/ml.

Genbank #

P13236

UniProt #

P13236

Background

Macrophage Inflammatory Protein-1 is a factor produced by macrophages that causes local inflammatory responses, and induces superoxide production by neutrophils . Two peptides are responsible for this activity, termed MIP-1-alpha, and MIP-1-beta. The two MIP proteins are the major factors produced by macrophages following their stimulation with bacterial endotoxins. Both proteins are involved in the cell activation of human granulocytes (neutrophils, eosinophils, and basophils) and appear to be involved in acute neutrophilic inflammation. Both forms of MIP-1 stimulate the production of reactive oxygen species in neutrophils and the release of lysosomal enzymes. They also induce the synthesis of other pro-inflammatory cytokines such as IL-1, IL-6 and TNF in fibroblasts and macrophages. MIP-1-alpha is a potent agonist of basophils, inducing a rapid change of cytosolic free calcium, the release of histamine and sulfido-leukotrienes, and chemotaxis. Murine MIP-1- alpha is the primary stimulator of TNF secretion by macrophages, whereas MIP- 1-beta antagonizes the inductive effects of MIP-1-alpha. In human monocytes, the production of MIP-1-beta can be induced by bacterial lipopolysaccharides and IL-7. The biological activities of MIP-1-alpha and MIP-1-beta are mediated by CCR5, a receptor that binds both factors. A second species of receptors for these two factors also appears to bind MCAF.

References

1. Invest. Ophthalmol. Vis. Sci., Apr 2009, 50: 4138.
2. Protein Eng. Des. Sel., Feb 2008, 21: 65 - 72.
3. J. Leukoc. Biol., Feb 2006, 79: 378 - 387.

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Human Macrophage Inflammatory Protein-1 beta Recombinant

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