Methyltransferase(s)

 

Methyltransferases are enzymes that transfer a methyl group from S-adenosylmethionine (SAM) to their substrates.DNA methyltransferases modify DNA by adding a methyl group to cytosines. DNA methylation of CpG dinucleotides suppresses gene expression and is critical for transcriptional silencing during imprinting, X-inactivation and other developmental processes. DNA methylation also plays a crucial role in the development of nearly all types of cancer.

Methyltransferases can also modify proteins; histone methyltransferases methylate lysines and arginines on histone side chains. Lysines may be mono-, di-, or trimethylated and arginines may be mono-, symmetrically or asymmetrically dimethylated. Each of these different ‘flavors’ of methylation can result in different chromatin conformations and gene expression levels.

Several histone methyltransferases have been linked to diseases, in particular cancer. The lysine methyltransferase EZH2 is a prime example of this. EZH2 is a methyltransferase that is part of the Polycomb Repressive complex (PRC2). EZH2, along with EED, RbAP48, SUZ12 and AEBP2, form a 5-member complex that methylates Lysine 27 of Histone H3 (H3K27). Abnormal expression of EZH2 has been linked to breast, prostate, endometrial, and other human cancers. Several point mutations in EZH2 cause it to hypermethylate H3K27, and are strongly associated with non-Hodgkins lymphomas and leukemias. Not surprisingly, inhibition of EZH2 is a key target for anti-cancer drug discovery. To aid in discovery, BPS offers a number of EZH2 mutant enzymes.

Aside from purified enzymes, BPS offers assay kits, inhibitors/activators, and substrates. The substrates offered by BPS include purified full-length histone proteins, tetramers, octamers, nucleosomes, and a number of modified histones. This diverse portfolio provides researchers with optimal flexibility in their experiments.

References:

1.  Vire, E. et al., Nature. 2006; 439: 871-874.
2.  Lyko, F. et al., J. Nat. Canc. Inst. 2005; 97(20): 1498-1506. 

 

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Catalog#SizeProduct NamePrice
27307 25 mg AMI-1 (sodium salt)
$335
52030 1 ml Anti- Adenosyl Homocysteine Antibody
$385
52140F 25 µl Anti-Dimethyl Histone H3 (Lys27) Monoclonal, Primary Antibody 6
$270
52140Q 25 µl Anti-JMJD1A, Primary Antibody 17
$195
52140E 40 μl Anti-JMJD2, Primary Antibody 5
$290
52140C-500 500 µl Anti-Trimethyl Histone H3 (Lys27) Monoclonal
$770
52140C-100 100 µl Anti-Trimethyl Histone H3 (Lys27) Monoclonal
$175
27107 50 mg Azacytidine (Vidaza)
$225
27220 10 mg BIX01294 (hydrochloride hydrate)
$220
52100 50 ml Blocking Buffer 1
$90
27221 1 mg Chaetocin
$230
52065 0.6 mg Core Histone Octamers
$450
50274 100 µg CoREST, His-tag
$440
27050 1 mg CTPB
$110
51101 10 µg DNMT1, GST-tag
$445
51100 10 µg DNMT1, His-FLAG-tags
$445
51102 10 µg DNMT2, GST-tag
$445
51103 10 µg DNMT3a, GST-tag
$445
51106 10 µg DNMT3A/DNMT3L Complex, His-tag, GST-tag
$460
51109

10 µg

DNMT3B/DNMT3L Complex, GST-tag, His-tag
$460
51104 10 µg DNMT3B/DNMT3L Complex, His-tag, GST-tag
$445
51005 50 µg DOT1L (KMT4), GST-tag
$375
50280 50 µg EED, FLAG-tag
$120
51020 50 µg EHMT1 (GLP), GST-tag
$425
27219 100 mg Ellagic acid
$100

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