EHMT1 (GLP), GST-tag Recombinant

Catalog #

51020

$440 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human euchromatic histone-lysine N-methyltransferase (EHMT1), also known as G9a-like protein, GLP or KMT1D, GenBank Accession No. NM_024757, a.a. 894-1298 (end) with N-terminal GST-tag, MW= 72.4 kDa, expressed in an E. coli expression system.

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Synonyms

EHMT1, GLP, KMT1D, G9a-like protein, euchromatic histone-lysine N-methyltransferase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

EHMT1 (GLP) inhibition curve using a chemiluminescent assay (#53007). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥53%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT

72.4 kDa

Amino Acids

894–1298 (end)

Specific Activity

≥122.3 pmole/min/µg

Genbank #

NM_024757

UniProt #

Q9H9B1

Tag(s)

N-terminal GST-tag

References

1. Huang, J., et al., J Biol. Chem. 2010 Mar 26; 285(13): 9636-41.
2. Ueda, J., et al., J Biol. Chem. 2006 Jul 21; 281(29): 20120-8.

Storage/Stability

>6 months at –80°C

Assay Conditions

50 μl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and EHMT1 (0.05 – 0.5 ng)) is added to substrate-coated wells on a Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only