SMYD2 (KMT3C) Chemiluminescent Assay Kit

Catalog #

51031

$725 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The SMYD2 Chemiluminescent Assay Kit is designed to measure SMYD2 activity for screening and profiling applications. The key to the SMYD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and the SMYD2 methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against SMYD2 (KMT3C)? Check out our Methyltransferase Screening Services.

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Synonyms

KMT3C

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

The SMYD2 Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 substrate, antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase

Materials Required But Not Supplied

TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog Number	Component	Amount	Storage
51014	SMYD2 human enzyme	80 µg	-80°C	*Avoid freeze/ thaw cycles!*
52120	100 µM S-adenosylmethionine	2 x 250 µl	-80°C
52140P2	Primary antibody 16-2	12.5 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
52193Z	4x HMT assay buffer 7	3 ml	-20°C
52100	Blocking buffer 4	50 ml	+4°C
79670	ELISA ECL Substrate A (translucent bottle)	6 ml	RT
79670	ELISA ECL Substrate B (brown bottle)	6 ml	RT
	White microplate precoated with histone substrate	1	+4°C

UniProt #

Q9NRG4

References

Brown MA, et al. Mol. Cancer 2006; 5:26.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See product datasheet for detailed assay protocol.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

Dry ice

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only