Methyltransferase(s)

 

Methyltransferases are enzymes that transfer a methyl group from S-adenosylmethionine (SAM) to their substrates.DNA methyltransferases modify DNA by adding a methyl group to cytosines. DNA methylation of CpG dinucleotides suppresses gene expression and is critical for transcriptional silencing during imprinting, X-inactivation and other developmental processes. DNA methylation also plays a crucial role in the development of nearly all types of cancer.

Methyltransferases can also modify proteins; histone methyltransferases methylate lysines and arginines on histone side chains. Lysines may be mono-, di-, or trimethylated and arginines may be mono-, symmetrically or asymmetrically dimethylated. Each of these different ‘flavors’ of methylation can result in different chromatin conformations and gene expression levels.

Several histone methyltransferases have been linked to diseases, in particular cancer. The lysine methyltransferase EZH2 is a prime example of this. EZH2 is a methyltransferase that is part of the Polycomb Repressive complex (PRC2). EZH2, along with EED, RbAP48, SUZ12 and AEBP2, form a 5-member complex that methylates Lysine 27 of Histone H3 (H3K27). Abnormal expression of EZH2 has been linked to breast, prostate, endometrial, and other human cancers. Several point mutations in EZH2 cause it to hypermethylate H3K27, and are strongly associated with non-Hodgkins lymphomas and leukemias. Not surprisingly, inhibition of EZH2 is a key target for anti-cancer drug discovery. To aid in discovery, BPS offers a number of EZH2 mutant enzymes.

Aside from purified enzymes, BPS offers assay kits, inhibitors/activators, and substrates. The substrates offered by BPS include purified full-length histone proteins, tetramers, octamers, nucleosomes, and a number of modified histones. This diverse portfolio provides researchers with optimal flexibility in their experiments.

References:

1.  Vire, E. et al., Nature. 2006; 439: 871-874.
2.  Lyko, F. et al., J. Nat. Canc. Inst. 2005; 97(20): 1498-1506. 

 

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Catalog#SizeProduct NamePrice
52035 96 reactions DNMT Universal Chemiluminescent Assay Kit
$1,325
52050L 96 reactions DNMT1 Chemiluminescent Assay Kit
$770
52033 96 reactions DNMT3A Chemiluminescent Assay Kit
$775
52034 96 reactions DNMT3B Chemiluminescent Assay Kit
$950
52202 96 reactions DOT1L (KMT4) Chemiluminescent Assay Kit
$660
53007 96 reactions EHMT1/GLP Chemiluminescent Assay Kit
$675
52079 96 reactions EZH1 Chemiluminescent Assay Kit
$1,400
52077 96 reactions EZH2 (A677G) Chemiluminescent Assay Kit
$1,400
52075 96 reactions EZH2 (Y641F) Chemiluminescent Assay Kit
$1,400
52076 96 reactions EZH2 (Y641N) Chemiluminescent Assay Kit
$1,400
52078 384 reactions EZH2 (Y641N) TR-FRET Assay Kit
$1,400
52009L 96 reactions EZH2 Chemiluminescent Assay Kit
$1,400
52059 384 reactions EZH2 Homogeneous Assay Kit
$2,800
52001L 96 reactions G9a Chemiluminescent Assay Kit
$675
52051 384 reactions G9a Homogeneous Assay Kit
$1,025
52072 96 reactions Histone H3(K9) Universal Methyltransferase New Assay Kit
$760
52074 96 reactions Histone H4(R3) Methyltransferase Assay Kit
$760
53008 96 reactions MLL1 Complex Chemiluminescent Assay Kit
$675
53010 96 reactions NSD1 Chemiluminescent Assay Kit
$820
53009 96 reactions NSD2 Chemiluminescent Assay Kit
$675
52004L 96 reactions PRMT1 Chemiluminescent Assay Kit
$675
52054 384 reactions PRMT1 Homogeneous Assay Kit
$1,025
52005L 96 reactions PRMT3 Chemiluminescent Assay Kit
$675
52055 384 reactions PRMT3 Homogeneous Assay Kit
$1,025
52041L 96 reactions PRMT4 Chemiluminescent Assay Kit
$675

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