Mouse Cot (MAP3K8), His-GST-Tags Recombinant
Catalog #
41050
$355
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Description
Mouse Cot kinase (MAP3K8), GenBank Accession No. NM_007746, a.a. 30-397, with N-terminal His-GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. Theoretical MW is 70 kDa.
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Synonyms
COT, TPL2, MEKK8, mitogen-activated protein kinase kinase kinase 8, Cancer Osaka Thyroid oncogene, Ewing Sarcoma Transformant, EST, Tumor Progression Locus 2, MAP3K8
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Product Data Gallery
10% SDS-PAGE Coomassie Staining
Product Info
Storage and Usage
Citations
Species
Mouse
Host Species/Expression System
Sf9 insect cells
Purity
≥38%
Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.4 mM KCl, 5 mM glutathione, 134 mM imidazole, 3 mM DTT and 20% glycerol.
MW
70 kDa (calculated)
Amino Acids
30–397
Specific Activity
8.5 pmole/min/mg
Genbank #
NM_007746
UniProt #
Q07174
Background
COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-κB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels.
References
1. Jeong, J.H. et al., PLoS One. 2011 Jan 18, 6(1):e16205.
2. Soria-Castro, I. et al. J Biol Chem. 2010 Oct 29,285(44):33805-15.
2. Soria-Castro, I. et al. J Biol Chem. 2010 Oct 29,285(44):33805-15.
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