MAT2A Assay Service
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Target
MAT2A
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Description
Screening and/or profiling inhibitor compounds against Methionine Adenosyltransferase 2A (MAT2A) activity in a biochemical assay.
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Synonyms
AdoMet Synthase 2
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Colorimetric
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Reference Compounds and IC50
FIDAS-5, 10 μM
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Assay Principle
The MAT2A Inhibitor Screening Assay is designed to measure MAT2A activity for screening and profiling applications. The MAT2A assay is performed in a 384-well format, with purified recombinant MAT2A enzyme, L-Methionine, ATP, MAT2A assay buffer, and colorimetric detection reagent. MAT2A catalyzes the formation of S-adenosylmethionine from methionine and ATP. Free phosphates from the reaction are measured using a colorimetric detection reagent.
Target Details
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Protein Family
Metabolic Enzyme
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UniProt
P31153
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Background
Methionine Adenosyltransferase 2A (MAT2A), also known as AdoMet Synthase 2, catalyzes the formation of S-adenosylmethionine from methionine and ATP. It is an important enzyme in cellular metabolism and is expressed in extrahepatic tissues. In liver, MAT2a expression is associated with growth, dedifferentiation, and cancer.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
