Human Leukemia Inhibitory Factor Recombinant

Catalog #

90208-A

$165 *

Size: 5 µg

Qty

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Description

LIF is a disulfide-linked monomer protein consisting of 181 amino acid residues, and migrates as an approximately 20 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human LIF mature chain was expressed in E. coli.

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Synonyms

LIF, HILDA,Cholinergic Differentiation Factor, CDF, Differentiation Inhibitory Activity, DIA, MLPLI, Leukemia Inhibitory Factor

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 4.5.

20 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

23–202

Biological Activity

The ED₅₀ was determined by the dose-dependent differentiation of M1 myeloid leukemic cells to be in the range of 0.01 ng/ml.

Genbank #

P15018

UniProt #

P15018

Background

LIF, a multifunctional, secreted glycoprotein that exists in both soluble and matrix-bound forms, displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is clear that LIF is related in both structure and mechanism of action to the interleukin (IL)-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130.

References

1. Endocrinology, Jun 2009, 150: 2915 - 2923.
2. Invest. Ophthalmol. Vis. Sci., Apr 2009, 50: 3880.
3. Invest. Ophthalmol. Vis. Sci., Apr 2009, 50: 682.

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Human Leukemia Inhibitory Factor Recombinant

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