MBP, His-Tag Recombinant

Catalog #

40535

$395 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human MBP (myelin basic protein), full-length encompassing amino acids 2-304 (end). This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

MBP, myelin basic protein, Myelin A1 Protein MBP, Myelin A1 protein

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Construct

MBP (His-2-304(end))

Host Species/Expression System

E. coli

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, variable imidazole

34 kDa

Amino Acids

2–304(end)

Genbank #

NM_001025101

UniProt #

P02686

Tag(s)

N-terminal His-tag

Background

MBP is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP is also present in the bone marrow and the immune system. Differential splicing events combined with post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. MBP is commonly used as a substrate for many kinases and methyltransferases.

References

1. Kishimoto, A., et al., J. Biol. Chem. 1985;260(23):12492-12499.
2. Quandt, J.A., et al., J. Immunol. 2012;189(6):2897-2908.

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Datasheets/Protocols

40535 Lot 210831-G3

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