ALK2 (ACVR1) Kinase Assay Kit

Catalog #

79605

$535 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The ALK2 (ACVR1) Kinase Assay Kit is designed to measure ALK2 (activin receptor-like kinase-2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ALK2 (amino acids 147-509), casein, ATP, and kinase assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against ALK2? Check out our Kinase Screening Services.

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Synonyms

Activin receptor-like kinase 2 (ACVRLK2), ACVRLK2, FOP, SKR1, TSRI, ALK

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Product Data Gallery

Inhibition of ALK2 kinase activity by LDN-212854

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40019	ALK2 (ACVR1), GST-Tag	10 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	100 µl	-20°C
79550	Casein (5 mg/ml)	100 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q04771

Background

ALK2 (activin receptor-like kinase-2), also known as ACVR1 (activin A receptor, type 1) is a protein of the BMP (bone morphogenetic protein) and TGFβ (transforming growth factor beta) family, and is a type I receptor. Activin signaling occurs by binding activin to heteromeric complexes of receptor type I and type II, with receptor type I being responsible for signaling and type II for ligand binding. SMAD1 (mothers against decapentaplegic homolog 1)/2/3/6 are activated by ALK2. This protein is involved in the development of several organs, such as heart, nervous system, and bone. Dysfunction of ALK2 can result in a set of rare diseases, namely fibrodysplasia ossificans progressive (FOP). ALK2 is also linked to DIPG (diffuse intrinsic pontine glioma). The development of ALK2 inhibitors has been hindered by the close similarity in the kinase domain of the 7 known ALK, with particular attention needed on inhibition of ALK5, which can result in cardiac toxicity. Clinical trials are ongoing and will pave the way for further studies using ALK2-specific inhibitors.

References

Cunha S. I. and Pietras K., 2011 Blood 117(26): 6999–7006.
Minsung K., et al., 2017 Biochemical and Biophysical Research Communications 492(1):121-127.
Rooney L. and Jones C., 2021 ACS Omega 6(32):20729-20734.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only