Mouse Interleukin-3 Recombinant
Catalog #
90189-A
$130
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Description
Recombinant mouse IL-3 is a disulfide-linked monomer consisting of 135 amino acid residues, and migrates as an approximately 15 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding murine Interleukin-3 mature chain was expressed in E. coli.
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Synonyms
IL-3, interleukin 3, Mast Cell Growth Factor, MCGF, IL3
Product Info
Storage and Usage
Citations
Species
Mouse
Host Species/Expression System
E. coli
Purity
≥98% by SDS-PAGE and HPLC
Format
lyophilized protein
Formulation
Lyophilized from a 0.2 µm filtered PBS solution.
MW
15 kDa
Endotoxin Level
Endotoxin level was found to be < 0.1 ng/μg (1 EU/μg), using the LAL gel clot method.
Biological Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells to be <0.05 ng/ml.
UniProt #
P01586
Background
IL-3 is produced mainly by T- cells following cell activation by antigens and mitogens, but also by keratinocytes, NK-cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial- derived recombinant IL-3 shows that glycosylation is not required for the activity of IL-3. IL-3 sequences are evolutionarily not well conserved. Human and murine IL-3 show approximately 29% homology at the protein level while murine and rat IL-3 show approximately 54% homology. IL-3-alpha and IL-3- beta are two isoforms of rat IL-3. IL-3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. IL-3/IL-3 receptor complexes have a Kds of 10-9 - 10-10 M. Binding of IL-3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein.
References
1. Mol. Cell. Biol., Apr 2009, 29: 1682 - 1693.
2. J. Biol. Chem., Aug 2008, 283: 23505 - 23509.
3. J. Biol. Chem., Jan 2009, 284: 2187 - 2193.
2. J. Biol. Chem., Aug 2008, 283: 23505 - 23509.
3. J. Biol. Chem., Jan 2009, 284: 2187 - 2193.
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