Human Interleukin-18 Recombinant

Catalog #

90183-A

$130 *

Size: 5 µg

Qty

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Description

Recombinant Interleukin-18 is a disulfide-linked monomer protein consisting of 158 amino acid residues, migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-18 mature chain was expressed in E. coli.

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Synonyms

Interleukin 18, Interferon-Gamma-Inducing Factor, IGIF, IL-18, IL18, IFN

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥98% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS, pH 7.

18 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

37–193

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of human PBMC co-stimulated with IL-12 cells was found to be in the range of 5 ng/ml.

Genbank #

Q14116

UniProt #

Q14116

Background

IL-18 is synthesized as a biologically inactive precursor protein that has limited biological activity. The proform of IL18 is processed by one of the caspases, Caspase-1, to generate the 18 kDa bioactive molecule. IL-18 is produced during the acute immune response by macrophages and immature dendritic cells. Interleukin-18 is expressed by a variety of immune and non-immune cells, including monocytes and macrophages. IL-18 is one of the pro-inflammatory cytokines. The activities of IL-18 appear to be species-specific. An important function of IL-18 is the regulation of functionally distinct subsets of T-helper cells required for cell mediated immune responses. IL-18 functions as a growth and differentiation factor for Th1 cells. IL-18 up-regulates FAS ligand mediated cytotoxic activitiy of murine natural killer cells. IL-18 is part of a complex regulatory circuit involved in causing cell death by apoptosis.

References

1. Tsutsui H, Nakanishi K. Immunotherapy. 2012 Dec,4(12):1883-94.
2. Barker BR, et al. Curr Opin Immunol. 2011 Oct,23(5):591-7

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Human Interleukin-18 Recombinant

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