Human IGFBP-3 Recombinant
Catalog #
90165-A
$130
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Description
Recombinant IGFBP-3 is a monomer protein consisting of 269 amino acid residues, migrates as an approximately 28 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human IGFBP-3 mature chain fused to an N-terminal His tag was expressed in CHO Cells.
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Synonyms
IGFBP-3, IGF-Binding Protein 3
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
CHO
Purity
≥95% by SDS-PAGE and HPLC
Format
lyophilized protein
Formulation
Lyophilized from 0.2 µm filtered PBS.
MW
28 kDa
Endotoxin Level
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity
The ED(50) was determined by the ability to inhibit IGF-II induced proliferation of MCF-7 and was found to be≤ 0.2 μg/ml in the presence of 15 ng/ml human IGF-II.
Genbank #
P17936
UniProt #
P17936
Background
IGFBPs are synthesized in the liver and are produced also by various tumor cell lines and cell types. IGFBPs are high affinity binding proteins for IGF. The major fraction of both types of IGF circulating in the blood are bound non-covalently to these carrier proteins. IGF binding proteins modulate IGF activities by increasing their plasma half lives and by inhibiting or promoting the interactions of IGF with receptors on certain target cells. In addition these binding proteins provide a reservoir for IGF in pericellular spaces. Some IGFBPs also have stimulating effects in vitro and some may inhibit the growth of cells. IGFBP1 is found predominantly in the placenta and the amniotic fluid. The predominant sites of IGFBP1 transcription in the human fetal kidney are those with most active differentiation. Elevated serum levels have been observed in patients with Laron-type dwarfism and Growth hormone deficiency. High serum levels of IGFBP1 are found in newborns and it has been suggested that this could be important in protecting them from hypoglycemia.
References
1. McCarthy, K., et al. Anticancer Research October 2009 vol. 29 no. 10 3785-3790.
2. Volzke, H., et al. Eur J Endocrinol November 1, 2009 161 705-713.
3. Paharkova-Vatchkova, V. And Lee, K-W. Endocr Relat Cancer June 1, 2010 17 293-302.
2. Volzke, H., et al. Eur J Endocrinol November 1, 2009 161 705-713.
3. Paharkova-Vatchkova, V. And Lee, K-W. Endocr Relat Cancer June 1, 2010 17 293-302.
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