Human EGFRvIII CHO K1 Cell Line (High or Low Expression)
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Catalog #
78145
As low as$7,150*
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Description
Recombinant hEGFRvIII-CHO K1 cell line stably expressing full-length human EGFRvIII receptor (accession number NP_001333870). Surface expression of hEGFRvIII was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hEGFRvIII expression (High and Low) to mimic different stages of cancer target cells with various EGFRvIII expression levels.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
The cell line has been screened to confirm the absence of Mycoplasma species.
Background
The epidermal growth factor receptor (EGFR, ErbB-1, HER1) belongs to a family of receptor tyrosine kinases that includes three other closely-related members (HER2, HER3 and HER4). EGFR gene amplification, mutation and re-arrangement are frequently observed in primary glioblastoma multiforme (GBM). The most common EGFR variant in GBM, EGFRvIII, is characterized by a deletion of 267 amino acids in the extracellular domain, leading to a receptor that is unable to bind ligand yet is constitutively active. EGFRvIII enhances the tumorigenic potential of GBM by activating and sustaining mitogenic, anti-apoptotic, and pro-invasive signaling pathways.
Storage/Stability
Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.
Culture Conditions
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Growth Medium 3B (BPS Bioscience, #79529): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 μg/ml of Hygromycin B to ensure the recombinant expression is maintained.
Applications
Screen for inhibitors of EGFRvIII signaling for immuno-oncology research and drug discovery.
Shipping Temperature
-80°C
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.