Human α Thrombin

Catalog #: 80111
Size: 1000 units
$195
Species: Human
MW: 37 kDa
Background:
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Description:
The Human Thrombin was activated from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. These activating enzymes were removed after activation. Complete activation was observed on SDS-PAGE. MW = 37 kDa.
UniProt
P00734
Synonym(s):
alpha thrombin, Coagulation factor II
Purity:
≥90% by SDS-PAGE
Supplied As:
Supplied as 0.33 mg total protein in 0.096 ml
Specific Activity:
3001 NIH units/mg 
Formulation:
50 mM sodium citrate, 0.2 M NaCl, 0.1% PEG-8000, pH 6.5
Purification:
Purified from human plasma that was tested and found negative by FDA-accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD (Creutzfeldt-Jakob Disease).
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Reference(s):
1. Spronk HM, et al. 2013. Curr Atheroscler Rep. 15(11):363.
2. Huntington JA. 2013. J Thromb Haemost. Suppl 1:254-64.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Purified Protein
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Product Datasheets

80111_Lot150731.pdf