Stable, recombinant HEK293 cell line expressing human TRPC3 (transient receptor potential cation channel, subfamily C, member 3, accession number NM_003305).
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
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Synonyms
TRPC3, Transient Receptor Potential Cation Channel, Subfamily C, Member 3
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Product Data Gallery
TRPC3 produced constitutive Ca2+ entry in the presence of 5M Gd3+ when expressed in HEK293. A) WT-HEK293 cells; B) TRPC3-HEK293 cells
Methacholine activated Ca2+ entry in TRPC3 expressed HEK293 cells. A) WT-HEK293 cells; B) TRPC3-HEK293 cells
Each vial contains ~1 X 106 cells in 1 ml of 10% DMSO.
Biological Activity
N-terminal FLAG tagged human TRPC3 channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of TRPC3 was characterized by calcium assay. TRPC3 produces a constitutive Ca2+ entry in HEK293 cells. Addition of methacholine, an activator of phospholipase C-linked muscarinic receptors, significantly activates TRPC3.
Genbank #
NM_003305
UniProt #
Q13507
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Background
TRPC3 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRP channels share a similar architecture of six-transmembrane domains and function as tetramers. Most channels are permeable to calcium and monovalent cations (Vassort et al., 2009). Specifically, TRPC3 channel belongs to a canonical TRP (TRPC) subfamily that is involved in calcium entry. TRPC3 is activated through phospholipase C-linked receptors. When expressed in HEK293 cells, TRPC3 behaves as a receptor activated channel with constitutive activity that cannot be further increased by store depletion (Trebak et al., 2002).
References
1. Albert A.P., Saleh S.N., and Large W.A. (2009). Current Medicinal Chemistry16: 1158-1165. 2. Lievremont J., et al. (2004). Am J Physiol Cell Physiol287: C1709-C1716. 3. Trebak M., et al. (2002). J. Biol. Chem.277: 21617-21623. 4. Vassort G. and Alvarez J. (2009). Can. J. Physiol. Pharmacol.87: 100-107.
Storage/Stability
Store in liquid nitrogen immediately upon receipt.
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS Cat. #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1B (BPS Cat. #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 μg/ml of Geneticin to ensure the recombinant expression is maintained. TRPC3-HEK293 cells should exhibit a typical cell division time of 24 hours.
Instructions for Use
See data sheet for detailed culturing and assay protocol.
Applications
• Monitor TRPC3 calcium channel activity • Screen for activators or inhibitors of TRPC3 calcium channel
Shipping Temperature
-80°C
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.
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