Histone H2b, Full Length, His-tag

Catalog #: 52022
Size: 1 mg
$450
Species: Human
Host Species: E. coli
MW: 14.6 kDa
Genbank #: NM_003528
Tag(s): N-terminal His-tag
a.a: 2–126(end)
Description:
Human Histone 2B, GenBank Accession No. NM_003528, a.a. 2-126(end) with N-terminal His-tag, MW = 14.6 kDa, expressed in an E. coli expression system.
UniProt
Q16778
Synonym(s):
H2B/c,H2BFC,HIST1H2BL,Histone H2B
Formulation:
50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol, 1 mM PMSF and 1 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.
Reference(s):

1.   Osley,  M.A.,  Brief  Funct. Genomic. Proteomic. 5(3):179-89 (2006). 
2.  Wyrick,  J.J.  and  Parra,  M.A.,  Biochim. Biophys Acta. 1789(1):37-44 (2009).   
3.   Emre, N.C.  and Berger, S.L., Cold Spring Harb  Symp  Quant  Biol.  69:289-99 (2004)

Application Reference(s):
Differential mode of antimicrobial actions of arginine-rich and lysine-rich histones against Gram-positive Staphylococcus aureus (2013)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase/Substrate
Product Type:
Recombinant Protein
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Product Datasheets

52022_Lot131219.pdf