Mouse EPHB1, GST-tag Recombinant

Catalog #

40199

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant mouse EPHB1 (a.a. 591-end), with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

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Synonyms

Elk, Net, Cek6, Elkh, Hek6, EPHT2, AW488255, 9330129L11, Ephrin Type B Receptor 2, EPHB-1

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

62 kDa

Amino Acids

591–end

Genbank #

NM_173447

Tag(s)

N-terminal GST-tag

Background

EPHB1 is a member of the Eph family of receptor tyrosine kinases. Ligand-activated EPHB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration (1). In addition, activated EPHB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. Expression of dominant-negative c-Src significantly reduced EPHB1-dependent ERK1/2 activation and chemotaxis (2).

References

1. Vindis, C. et al: EphB1-mediated cell migration requires the phosphorylation of paxillin at Tyr-31/Tyr-118. J Biol Chem. 2004 Jul 2;279(27):27965-70.
2. Vindis, C. et al: EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis. J Cell Biol. 2003 Aug 18;162(4):661-71.

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Mouse EPHB1, GST-tag Recombinant

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