Recombinant human Elane (Neutrophil Elastase), encompassing amino acids 28-252. The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The recombinant protein was affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration. The protein appears as two separate bands in SDS PAGE due to slightly different glycosylation patterns. The identity of the protein has been confirmed by protein sequencing (by Edman degradation) of the N-terminus. Both bands are recognized by an anti-His antibody, indicating that the C-terminus is intact.
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
MW
27 kDa + glycans
Amino Acids
28-252
Glycosylation
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Aggregation
<10%
Genbank #
NM_001972
UniProt #
P08246
Tag(s)
C-terminal Avi-His-tag
Storage/Stability
At least 6 months at -80°C.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.