CD95 CHO Cell Line (Medium or High Expression)

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Catalog #

78499

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Description

The CD95 CHO cell line is a recombinant clonal stable CHO cell line constitutively expressing full-length human CD95 receptor (accession number: NM_000043.3). Surface expression of human CD95 was confirmed by flow cytometry. Each clonal cell line was selected for different levels of CD95 expression (High or Medium) to mimic varying CD95 expression levels in cancer cells.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

FAS, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptor superfamily member 6

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Product Data Gallery

Flow cytometry analysis of cell surface expression of human CD95 in CHO-K1 cells

Effects of ELANE on Caspace3/7 Pathway Activation

Effects of rhELANE on CD95 CHO cell line (High Expression Only)

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3B	BPS Bioscience #79529

Materials Required for Cellular Assay

Name	Ordering Information
F-12K Medium (Kaighn’s Modification of Ham’s F-12 Medium)	ThermoFisher #21127030
ELANE (Elastase), Avi-His-Tag HiP™ Recombinant	BPS Bioscience #101141
Recombinant Mouse Active Cathepsin C/DPPI Protein, CF	R&D Systems #2336-CY-010
96-well Flat Clear Bottom White Polystyrene TC-treated Microplates	Corning #3610
Caspase-Glo® 3/7 Assay System	Promega #G8091

Genbank #

NM_000043.3

UniProt #

P25445

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

CD95 (Fas, APO-1, TNFRSF6) is a transmembrane cell-surface receptor with multifunctional regulatory effects in apoptosis and proliferation regulation. In some cellular contexts, interactions with the receptor ligand (CD95L) have been shown to activate JNK, ERK1, and NF-kB pathways to induce cell proliferation. In other cases, ligand interactions can trigger the release of a cytoplasmic death domain and induce apoptosis. This function is used by the immune system to kill cancer cells, and consequently, cancer cells can become resistant to CD95-induced apoptosis. The overexpression of CD95 in this cell line allows for the rapid testing of novel therapeutics that aim to increase CD95-mediated apoptosis in cancer cells.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3B (BPS Bioscience #79529): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 µg/ml of Hygromycin B.

Media Required for Functional Cellular Assay: F-12K medium (ThermoFisher #21127030)

Applications

Test compounds that activate the death domain of CD95 in cancer cells.
Screen and validate antibodies against CD95 for drug discovery.
Design CD95 binding assays to screen for CD95 ligands.

Shipping Temperature

Dry ice

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only