EIF2AK2 Kinase Assay Kit

Catalog #

78433

$560 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The EIF2AK2 Kinase Assay Kit is designed to measure EIF2AK2 kinase activity for screening and profiling applications using ADP-Glo^® as a detection reagent.

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Synonyms

Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, Protein kinase RNA-activated

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The assay kit comes in a convenient 96-well format, with enough purified recombinant EIF2AK2 kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo^® Kinase Assay (Promega #V6930)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
40058	EIF2AK2*	1.5 µg	-80°C
79334	Kinase assay buffer 1 (5x)	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40535	Myelin basic protein (MBP), 5 mg/ml	100 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube

UniProt #

P19525

Background

EIF2AK2 (eukaryotic translation initiation factor 2 alpha kinase 2, also known as PKR) is a protein kinase shown to be involved in HIV/gp120-associated neurodegeneration.¹EIF2AK2 acts as a critical mediator of gp120 neurotoxicity and is a substrate for a family of protein kinases that respond to various forms of environmental stress. EIF2AK2 plays a critical role in mRNA translation, cell proliferation and apoptosis. A novel cross-talk between the EIF2AKs and p53 may have implications in cell proliferation and tumorigenesis.²

References

Baltzis, D. et al: The eIF2alpha kinases PERK and PKR activate glycogen synthase kinase 3 to promote the proteasomal degradation of p53. J. Biol Chem. 2007; 282(43):31675-87.
Alirezaei, M. et al: Human immunodeficiency virus-1/surface glycoprotein 120 induces apoptosis through RNA-activated protein kinase signaling in neurons. J. Neurosci. 2007;27(41):11047-55.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only