Demethylase(s)

 

Demethylases are enzymes that remove methyl groups from lysine and arginine residues of proteins, the most commonly studied being histone demethylases. Methylation of histones is essential for regulating transcription and maintaining chromatin structure, and demethylases, like all histone modifying enzymes, play an important role in development and gene regulation. All of the demethylases, with the exception of LSD1, contain a catalytic jumonji domain. Many of these enzymes require a cofactor such as FAD, Fe(II), or α-ketoglutarate.

Like, methyltransferases, demethylases exhibit a high level of substrate specificity with respect to their target lysine. In addition, they are sensitive to the specific type of methylation that they recognize and subsequently demethylate. For example, some demethylases are only capable of demethylating mono- and dimethyl substrates, whereas others are capable of demethylating all states of lysine methylation.

Given the critical role that demethylases play in gene regulation, it is not surprising that they have been linked to many human cancers. BPS was the first company to supply active, high purity histone demethylases for life science research. We provide a unique and comprehensive offering of enzymes, assay kits, and inhibitors/activators to meet all of your research needs. Our large portfolio of substrates, which includes and a number of modified histones, makes it easy to study histone demethylase substrate specificity.

References:

1.  Pedersen, M.T., et al., Trends Cell Biol. 2010; 20(11): 662-671.

 

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Catalog#SizeProduct NamePrice
50098 10 ml 2x LSD1 Buffer 1
$200
50099 10 ml 3x LSD1 Buffer 2
$200
52301 10 ml 4x DMT Detection Buffer 1
$200
52406 10 ml 4x HDM Assay Buffer 1
$200
52407 10 ml 4x HDM Assay Buffer 2
$200
52408 10 ml 4x HDM Assay Buffer 3
$200
52409 10 ml 4x HDM Assay Buffer 4
$200
52410 10 ml 4x HDM Incomplete Assay Buffer
$200
79659 96 reactions ALKBH5 Chemiluminescent Assay Kit
$660
100057 20 µg ALKBH5, FLAG-Tag
$440
52140F 25 µl Anti-Dimethyl Histone H3 (Lys27) Monoclonal, Primary Antibody 6
$280
52140Q 25 µl Anti-JMJD1A, Primary Antibody 17
$200
52140E 40 μl Anti-JMJD2, Primary Antibody 5
$300
79556 50 ml Blocking Buffer 1
$95
50157 96 reactions FBXL10 (KDM2B) Chemiluminescent Assay Kit
$680
50610 384 reactions FBXL10 (KDM2B) Homogeneous Assay Kit
$1,080
79676 96 Reactions FBXL10 (KDM2B) Homogeneous Assay Kit
$680
50120 20 µg FBXL10 (KDM2B, JHDM1B), FLAG-tag
$475
50611 384 reactions FBXL11 (KDM2A) Homogeneous Assay Kit
$1,080
50156 20 µg FBXL11 (KDM2A, JHDM1A), FLAG-Avi-tags
$475
79344 96 reactions FTO Chemiluminescent Assay Kit
$660
79306 100 µg FTO, His-Tag (E. coli derived)
$465
79480 20 µg FTO, His-Tag (Sf9 derived)
$465
27627-2 50 mg GSK J4 HCl
$445
27627-1 10 mg GSK J4 HCl
$175

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