Demethylase(s)

 

Demethylases are enzymes that remove methyl groups from lysine and arginine residues of proteins, the most commonly studied being histone demethylases. Methylation of histones is essential for regulating transcription and maintaining chromatin structure, and demethylases, like all histone modifying enzymes, play an important role in development and gene regulation. All of the demethylases, with the exception of LSD1, contain a catalytic jumonji domain. Many of these enzymes require a cofactor such as FAD, Fe(II), or α-ketoglutarate.

Like, methyltransferases, demethylases exhibit a high level of substrate specificity with respect to their target lysine. In addition, they are sensitive to the specific type of methylation that they recognize and subsequently demethylate. For example, some demethylases are only capable of demethylating mono- and dimethyl substrates, whereas others are capable of demethylating all states of lysine methylation.

Given the critical role that demethylases play in gene regulation, it is not surprising that they have been linked to many human cancers. BPS was the first company to supply active, high purity histone demethylases for life science research. We provide a unique and comprehensive offering of enzymes, assay kits, and inhibitors/activators to meet all of your research needs. Our large portfolio of substrates, which includes and a number of modified histones, makes it easy to study histone demethylase substrate specificity.

References:

1.  Pedersen, M.T., et al., Trends Cell Biol. 2010; 20(11): 662-671.

 

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Catalog#SizeProduct NamePrice
50610 384 reactions FBXL10 (KDM2B) Homogeneous Assay Kit
$1,050
50611 384 reactions FBXL11 (KDM2A) Homogeneous Assay Kit
$1,050
50510 384 reactions JARID1A (KDM5A) Homogeneous Assay Kit
$1,950
50512 384 reactions JARID1B (KDM5B) Homogeneous Assay Kit
$1,950
50511 384 reactions JARID1C (KDM5C) Homogeneous Assay Kit
$1,950
50420 384 reactions JHDM1D (KDM7A) Homogeneous Assay Kit
$1,725
50412 384 reactions JMJD1A (KDM3A) Homogeneous Assay Kit
$1,950
50109 96 reactions LSD1 (KDM1A) Chemiluminescent Assay Kit
$435
50107 384 reactions LSD1 (KDM1A) Fluorescent Assay Kit
$565
50106 96 reactions LSD1 (KDM1A) Fluorescent Assay Kit
$435
50108 384 reactions LSD1 (KDM1A) Homogeneous Assay Kit
$540
50098 10 ml 2x LSD1 Buffer 1
$185
50099 10 ml 3x LSD1 Buffer 2
$185
52301 10 ml 4x DMT Detection Buffer 1
$185
52406 10 ml 4x HDM Assay Buffer 1
$185
52407 10 ml 4x HDM Assay Buffer 2
$185
52408 10 ml 4x HDM Assay Buffer 3
$185
52409 10 ml 4x HDM Assay Buffer 4
$185
52410 10 ml 4x HDM Incomplete Assay Buffer
$185
52140F 25 µl Anti-Dimethyl Histone H3 (Lys27) Monoclonal, Primary Antibody 6
$270
52140Q 25 µl Anti-JMJD1A, Primary Antibody 17
$195
52140E 40 μl Anti-JMJD2, Primary Antibody 5
$290
52100 50 ml Blocking Buffer 1
$80
50157 96 reactions FBXL10 (KDM2B) Chemiluminescent Assay Kit
$640
50120 20 µg FBXL10 (KDM2B, JHDM1B), FLAG-tag
$450

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