Anti–JMJD2a polyclonal antibody

Catalog #

25296

$335 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a KLH–conjugated synthetic peptide containing a sequence from the central part of the protein.

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Synonyms

JHDM3A, KDM4A, JMJD2

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Affinity purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300

Concentration

50 µg/76 µl

Immunogen

synthetic peptide

Background

JMJD2a (UniProtKB/Swiss–Prot entry O75164), belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono– and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F–responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human JMJD2a (Cat. # 25296), crude serum and flow-through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.

Western blot analysis using the antibody directed against JMJD2a
Nuclear extracts from HeLa cells (40 µg) were analyzed by Western blot using the antibody against JMJD2a (Cat. # 25296) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.

Applications

ELISA (1:100 – 1:500)
WB (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–JMJD2a polyclonal antibody

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