COX2, FLAG-His-Tags Recombinant

Catalog #

71111

$570 *

Size: 100 µg

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Description

Recombinant human COX-2, full length encompassing amino acids 1-604 (end). This construct contains a C-terminal FLAG followed by an His-tag (6xHis). This protein was affinity purified.

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Synonyms

cox cox1 cox 1 cox 2 cox2 Cyclooxygenase-2 Prostaglandin-Endo-peroxide Synthase 2, Prostaglandin G/H Synthase and Cyclooxygenase, PTGS2, Prostaglandin H2 Synthase, PHS-2, HCox-2, Inducible Prostaglandin H Synthase 2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

COX2 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

COX-2 (1-604(end)-FLAG-His)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide, 300 µM diethyldithiocarbamate and 0.08% Triton X-100.

71 kDa

Amino Acids

1-604 (end)

Genbank #

NM_000963

UniProt #

P35354

Tag(s)

C-terminal FLAG-His-Tags

Background

COX-2, also known as prostaglandin-endoperoxidase synthase 2, PTGS2 or cyclooxygenase-2, is a homodimer involved in inflammation processes. It is involved in the conversion of arachidonic acid (AA) to prostaglandin H (PGH2), which can then be converted into prostacyclin, prostaglandins or thromboxane A2. Expression of this enzyme is only found during inflammation. The absence of COX-2 in normal tissues makes it a suitable target for inhibitor-based therapies in inflammation. Inhibitors of COX-2 fall under the NSAID (nonsteroidal anti-inflammatory drugs) class and are used to treat several forms of arthritis and sport injuries. However, it was found that current inhibitors can increase the chance of cardiovascular pathologies, such as heart failure and stroke. Further studies are required to identify the mechanisms involved in this serious side effect and develop new inhibitors.

References

1. Kosaka, T., et al., Eur J Biochem. 1994 May 1;221(3):889-97.
2. Cox, D.G., et al., Br J Cancer. 2004 Jul 19;91(2):339-43.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Activity was measured by titrating the enzyme in Assay buffer (100 mM Tris-HCl, pH 8, 3 µM EDTA) with 100 µM Ampliflu™ Red and 50 µM arachidonic acid (Cayman Chemical #90010.1 peroxide free) and measuring fluorescence (λex 535; λem 590) after 5 min. Reaction volume was 100 µl.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes

Regulatory Status

Research Use Only

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Datasheets/Protocols

71111 Lot 230804

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COX2, FLAG-His-Tags Recombinant

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