Chemi-Verse™ CDK8/Cyclin C Kinase Assay Kit

Catalog #

78886

$850 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ CDK8/Cyclin C Kinase Assay is a luminescent assay designed to measure CDK8 (cyclin-dependent kinase 8)/Cyclin C kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant complex of CDK8/Cyclin C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

CDK8, cyclin dependent kinase 8, IDDHBA, Cell division protein kinase 8, Mediator complex subunit CDK8, Protein kinase K35, K35, CCNC, cyclin C, CycC, SRB11 homolog, SRB11, hSRB11, CDK-8

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Product Data Gallery

Inhibition of CDK8/Cyclin C kinase activity by Dinaciclib

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
100433	CDK8/Cyclin C, GST-Tag Recombinant*	15 µg	-80°C
79334	5x Kinase Assay Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
79604	CDK Substrate Peptide 2	125 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

CDK8: P49336: CyclinC: P24863

Background

CDK8 (cyclin dependent kinase 8) is a member of the cyclin-dependent protein kinase family involved in cell cycle regulation, transcription and splicing. CDK associates with cyclins, which induce a conformational change that results in a dramatic increase of the kinase activity. Cyclin levels vary during the cell cycle, which allows cyclins to regulate CDK activity in the cell. Dissociation of the complex returns CDK to its basal activity, and CDK is degraded by ubiquitin mediated proteolysis. In addition to cyclin, CDK8 is part of a Mediator complex that includes CDK19, MED12 and MED13 and acts as a negative regulator of RNA Polymerase II. CDK8 regulates several pathways, such as Notch, Wnt/β-catenin and Sonic Hedgehog (Shh) and has been described as an oncogenic driver and implicated in several cancer types (breast, ovarian, gastric cancer and leukemia). The understanding of the mechanisms involved in cell cycle regulation, and its control via the use of small molecule inhibitors will open new therapeutic avenues for the treatment of cancer.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only