Chemi-Verse™ CDK18/Cyclin Y Kinase Assay Kit

Catalog #

78888

$850 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ CDK18/Cyclin Y Kinase Assay is a luminescent assay designed to measure CDK18 (cyclin-dependent kinase 18)/Cyclin Y kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant CDK18/Cyclin Y complex, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

Cell division protein kinase 18, PCTAIRE-motif protein kinase 3, Serine/threonine-protein kinase PCTAIRE-3, PCTAIRE3, PCTK3, Cyclin-dependent kinase 18, C10orf9, CBCP1, CFP1, Cyclin box protein 1, Cyclin fold protein 1, cyclin-X, CDK-18

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Product Data Gallery

Inhibition of CDK18/Cyclin Y kinase activity by Dinaciclib.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
100608	CDK18/Cyclin Y, GST-Tag Recombinant*	10 µg	-80°C
79334	5x Kinase Assay Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	Rb Protein (2 mg/ml)	125 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

CDK18: Q07002; Cyclin Y: Q8ND76

Background

CDK18 (cyclin dependent kinase 18) is a member of the cyclin-dependent protein kinase family involved in cell cycle, transcription and splicing. CDK associates with cyclins, which induce a conformational change that results in a dramatic increase of kinase activity. Cyclin levels vary during the cell cycle, allowing cyclins to regulate CDK activity in the cell. Dissociation of the complex returns CDK to its basal activity, and CDK is degraded by ubiquitin mediated proteolysis. Lack of regulation in cell cycle can result in apoptosis and finally in cancer, neurodegenerative diseases (Alzheimer’s or Parkinson disease) and cardiovascular complications (stroke). The understanding of the mechanisms involved in cell cycle regulation, and its control via the use of small molecule inhibitors will open new therapeutic avenues for the treatment of cancer and neurodegenerative diseases.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ CDK18/Cyclin Y Kinase Assay Kit

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