CDK8/Cyclin C, GST-tags Recombinant
Catalog #
100433
$410
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Description
Complex of CDK8 (cyclin dependent kinase 8) and cyclin C (also known as CCNC).
Recombinant human CDK8, full length encompassing amino acids 2-464 (end), contains an N-terminal GST-tag. Recombinant human Cyclin C, full length encompassing amino acids 2-283 (end), also contains an N-terminal GST-tag. The two proteins were co-expressed in Sf9 insect cells and spontaneously formed a complex, which was affinity purified.
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Synonyms
CDK8, cyclin dependent kinase 8, IDDHBA, Cell division protein kinase 8, Mediator complex subunit CDK8, Protein kinase K35, K35, CCNC, cyclin C, CycC, SRB11 homolog, SRB11, hSRB11, CDK-8
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Product Data Gallery
4-20% SDS-Page Coomassie Staining
Product Info
Storage and Usage
Citations
Species
Human
Construct
CDK8 (GST-2-464(end)) / Cyclin C (GST-2-283(end))
Host Species/Expression System
Coexpressed in Sf9
Purity
≥90%
Supplied As
Aqueous buffer solution.
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol.
MW
CDK8: 79 kDaCyclinC: 59 kDa
Amino Acids
CDK8: 2-464(end)CyclinC: 2-238(end)
Genbank #
CDK8: NM_001260CyclinC: NM_005190
UniProt #
CDK8: P49336CyclinC: P24863
References
1. Akoulitchev S, et al. Nature 407: 102-106, 2000.
2. Firestein R, et al. Nature 455: 547-551, 2008.
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