CECR2, GST-Tag Recombinant

Catalog #

31138

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human bromodomain CECR2 (cat eye syndrome chromosome region, candidate 2), encompassing amino acids 430-543, which correspond to the single bromodomain. This construct contains an N-terminal GST-tag. This protein was affinity purified.

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Synonyms

CECR2, cat eye syndrome chromosome region, candidate 2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CECR2 Binding

CECR2 inhibition curve using a TR-FRET assay (#32622). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.

40 kDa

Amino Acids

430–543

Genbank #

NM_031413

UniProt #

Q9BXF3

Tag(s)

N-terminal GST-tag

Background

CECR2 (cat eye syndrome chromosome region candidate 2) is an epigenetic factor containing a bromodomain able to recognize acetylated lysines. It is involved in DNA Damage Responses (DDR), neurulation, spermatogenesis, and reprogramming. It has been linked to cancer and was identified as an epigenetic driver of metastatic breast cancer. It can form a complex with RELA (v-rel avian reticuloendotheliosis viral oncogene homolog A), increasing the access to chromatin of EMT (epithelial-mesenchymal transition) related genes and NF-κB (nuclear factor kappa B) target genes. The use of CECR2 inhibitors, such as GNE-886 and NVS-CECR2-1, is a promising area in cancer therapy.

References

1. Chen, J., et al., Int J Dev Biol. 2010; 54(5):925-9.
2. Lee SK, et al., Mol Cells. 2012 Jul; 34(1):85-91.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to CECR2 TR-FRET Assay Kit (BPS Bioscience #32622) with various concentrations of CeCR2.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Bromodomain

Regulatory Status

Research Use Only