CDK18/Cyclin Y, GST-tags Recombinant

Catalog #

100608

$465 *

Size: 10 µg

Qty

Bulk/Custom

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Description

CDK18/Cyclin Y complex of human CDK18, also known as PCTK3 and PCTAIRE3, GenBank Accession No. BC011526, a.a. 1-474(end) with N-terminal GST-tag, MW=76 kDa, and human Cyclin Y, also known as CCNY, CBCP1, CCNX, and CFP1, GenBank Accession No. NM_145012, a.a. 2-341(end) with N-terminal GST-tag. MW= 64 kDa. Co-expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

CDK18: PCTK3, PCTAIRE3, CDK18
Cyclin Y: CCNY, CBCP1, CCNX, CFP1, CDK-18

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Supplied As

Aqueous buffer solution.

Formulation

50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

CDK18: 76 kDa
Cyclin Y: 64 kDa

Amino Acids

CDK18: 1-474end)
Cyclin Y: 2-341(end)

Specific Activity

≥35 pmol/μg/min

Genbank #

CDK18: BC011526
Cyclin Y: NM_145012

UniProt #

CDK18: Q07002
Cyclin Y: Q8ND76

Tag(s)

CDK18: N-terminal GST-tag
Cyclin Y: N-terminal GST-tag

References

1. Meyerson, M. et al, EMBO J. 11: 2909-2917, 1992.
2. Okuda, T. et al, Oncogene 7: 2249-2258, 19.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

5 mM MOPS, 2.5 mM βglycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 50 ng/ml BSA, 2 mM ATP, 200 µg of MBP (or Histone H1) as substrate. Add [33P]-ATP and incubate at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only