CDK17/Cyclin Y, GST-tags Recombinant

Catalog #

100606

$465 *

Size: 10 µg

Qty

Bulk/Custom

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Description

CDK17/Cyclin Y complex of human CDK17, also known as PCTK2, PCTAIRE2, GenBank Accession No. NM_002595, a.a. 1-523(end) with N-terminal GST-tag, MW=88 kDa, and human Cyclin Y, also known as CCNY, CBCP1, CCNX, and CFP1, GenBank Accession No. NM_145012, a.a. 2-341(end) with N-terminal GST-tag. MW= 64 kDa. Co-expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

CDK17: PCTK2, CDK17, PCTAIRE2
Cyclin Y: CCNY, CBCP1, CCNX, CFP1, CDK-17

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Supplied As

Aqueous buffer solution.

Formulation

50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

CDK17: 88 kDa
Cyclin Y: 64 kDa

Amino Acids

CDK17: 1-523(end)
Cyclin Y: 2-341(end)

Specific Activity

≥20 pmol/μg/min

Genbank #

CDK17: NM_002595
Cyclin Y: NM_145012

UniProt #

CDK17: Q00537
Cyclin Y: Q8ND76

Tag(s)

CDK17: N-terminal GST-tag
Cyclin Y: N-terminal GST-tag

References

1. Meyerson, M. et al, EMBO J. 11: 2909-2917, 1992.
2. Hirose T. et al, Eur J Biochem. 1997 Oct 15;249(2):481-8.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 50 ng/ml BSA, 2 mM ATP, 200 μg of MBP (or Histone H1) as substrate. Add [33P]-ATP and incubate at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only