c-Met Kinase Assay Kit

Catalog #

79559

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The c-Met Kinase Assay Kit is designed to measure c-Met kinase activity for screening and profiling applications using Kinase-Glo^® MAX as a detection reagent. The recombinant protein used in the kit corresponds to amino acids 956-1390 of c-Met, containing the tyrosine kinase domain.

Need us to run inhibitor screens or profile your compounds against c-Met? Check out our Kinase Screening Services.

This product has been cited 2 times.

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Synonyms

Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, MET

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Product Data Gallery

Product Info

Storage and Usage

Citations2

Assay Kit Format

Luminescent

Supplied As

The assay kit comes in a convenient 96-well format, with enough purified recombinant c-Met kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40255	c-Met, GST-tag*	2.5 µg	-80°C	*Avoid multiple freeze/ thaw cycles!*
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40217	PTK substrate, Poly (Glu:Tyr, 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

*The concentration of the protein is lot-specific and will be indicated on the tube

UniProt #

P08581

Background

c-Met, also known as HGFR (hepatocyte growth factor receptor), is a tyrosine kinase receptor encoded by the gene MET. Upon binding its ligand HGF (hepatocyte growth factor), c-Met activates multiple cellular processes including proliferation, adhesion and angiogenesis. Importantly, c-Met is overexpressed in various carcinomas, suggesting that HGF/c-Met signaling pathway could be a promising target for cancer treatment.

References

Recondo G. et al. Targeting MET Dysregulation in Cancer. Cancer Discov. 2020; 10(7): 922-934. Review.

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide

Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/ thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only