BTK(C481F), His-Tag Recombinant

Catalog #

101040

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human BTK (Bruton Tyrosine Kinase), full length, containing mutation (C481F). The N-terminal end of the protein is fused to a His-tag. The protein was affinity purified.

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Synonyms

AGMX1, AT, ATK, BPK, IMD1, PSCTK1, XLA, Bruton tyrosine kinase, IGHD3

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

BTK(C481F)(His-2-658(end))

Mutation

C481F

Host Species/Expression System

Sf9 insect cells

Purity

70%

Supplied As

Aqueous buffer solution.

Formulation

50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 DTT, 25% glycerol.

75 kDa

Amino Acids

2-658(end)

Specific Activity

2.2 pmol/min/μg

Genbank #

NM_000061

UniProt #

Q06187

Tag(s)

C-terminal His-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

The kinase assay is performed using the ADP-Glo™ Kinase Assay kit (Promega; Cat# V9101) which quantifies the amount of ADP produced. The ADP-Glo™ Reagent is added to terminate the reaction and to deplete the remaining ATP. The Kinase Detection Reagent is then added to convert ADP to ATP and to measure the newly synthesized ATP using a luciferase reaction.

Assay: kinase activity was measured using a Poly-Glu/Tyr(4:1) substrate. The reaction was initiated by mixing Increasing amounts of the protein kinase with 25 μM ATP in 40 mM Tris-HCl, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, 250 μM DTT, and 0.2 mg/ml Poly-Glu/Tyr. The reaction was terminated by addition of the ADP-Glo™ Reagent, and the Kinase Detection Reagent was added. Phosphorylation was measured by detection of luminescence. The blank was determined from a “no kinase” sample.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

101040 Lot 210421

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BTK(C481F), His-Tag Recombinant

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