Human Brain-Derived Neurotrophic Factor Recombinant

Catalog #

90110-A

$130 *

Size: 2 µg

Qty

Bulk/Custom

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Description

Recombinant BDNF is a disulfide-linked homodimeric protein consisting of two 119 amino acid residues. migrates as an approximately 27 kDa protein under non-reducing conditions and as a 13-14 kDa protein under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Brain Derived Neurotrophic Factor mature chain was expressed in E. coli.

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Synonyms

Brain-Derived Neurotrophic Factor, BDNF, Abrineurin

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered citric acid, pH 5.7.

14 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

129–247

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of rat C6 cells, and was found to be in the range of 0.5 µg/ml.

Genbank #

P23560

UniProt #

P23560

Background

BDNF is found in neurons of the central nervous system. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. The biological activity of BDNF is mediated by a receptor that belongs to the trk family of receptors encoding a tyrosine-specific protein kinase. BDNF only binds weakly to the gp140trk receptor (to which NGF binds with high affinity), and it binds to the NGF receptor known as LNGFR. BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. The factor supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neurons in vitro. BDNF does not appear to act on sympathetic ganglia. In specific neurons of the central nervous system located in the hippocampus and the cortex the synthesis of BDNF is influenced by neuronal activity either positively (glutamate transmitter system) or negatively (GABA transmitter system).

References

1. PNAS, Sep 2009, 106: 16481 - 16486.
2. J. Neurosci., Sep 2009, 29: 11650 - 11661.

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Human Brain-Derived Neurotrophic Factor Recombinant

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