The BCL-XL TR-FRET Assay Kit is designed to measure the inhibition of BCLXL binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL-XL, peptide ligand, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against BCL-XL? Check out our Apoptosis Screening Services.
●
Synonyms
Bcl-Xlong, B cell lymphoma XL, HTRF, BCLXL, BCL2L1
Lessene, G., et al., Nature Chem. Biol. 2013 ; 9:390
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol
Applications
Useful for screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
-80°C (dry ice)
Notes
Since January of 2018, this kit has been optimized for better performance. The previous version of the BCL-XL TR-FRET Assay Kit, Cat. #50223, can still be purchased upon special request.
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
Powered by Bioz
