Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST-tag, MW=147 kDa, expressed in baculovirus expression system.
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT
MW
147 kDa
Amino Acids
full length
Specific Activity
≥300 pmole/min/μg.
Genbank #
NM_001096
UniProt #
P53396
Tag(s)
N-terminal GST-tag
Background
ATP citrate-lyase is an important enzyme for cytosolic acetyl-CoA synthesis. During fatty acid synthesis, Citrate and CoA form acetyl-CoA and oxaloacetate.
ATP citrate-lyase has emerged as new target for cancer treatment. Studies have found that many types of cancer including glioblastoma, colorectal cancer, breast cancer, non-small cell lung cancer, and hepatocellular carcinoma experience abnormally high levels of ATP citrate-lyase.
References
1. Migita T. et al., Cancer Res. 68 (20), 8547- 8554 (2008). 2. Ramakrishna S. et al., Biochemistry 29 (33), 7617-7624 (1990).
Storage/Stability
>6 months at –80°C
Assay Conditions
A 25 µl ACLY reaction is conducted in a buffer
containing 40 mM Tris (pH 8.0), 10 mM MgCl2,
2 mM DTT, 2 mM potassium citrate, 0.1 mM CoA
and 6-100 ng human ACLY at 30 oC for 60 min.
Enzyme activity was assayed using ADP-Glo
reagents (Promega)
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.