ATP Citrate Lyase, GST-tag Recombinant

Catalog #

50255

$540 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST-tag, MW=147 kDa, expressed in baculovirus expression system.

This product has been cited 2 times.

●

Synonyms

ATP citrate lyase, ACLY, ACL

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

ACLY inhibition curve using a luminescent assay (#79904). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥68%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT

147 kDa

Amino Acids

full length

Specific Activity

≥300 pmole/min/μg.

Genbank #

NM_001096

UniProt #

P53396

Tag(s)

N-terminal GST-tag

Background

ATP citrate-lyase is an important enzyme for cytosolic acetyl-CoA synthesis. During fatty acid synthesis, Citrate and CoA form acetyl-CoA and oxaloacetate.

ATP citrate-lyase has emerged as new target for cancer treatment. Studies have found that many types of cancer including glioblastoma, colorectal cancer, breast cancer, non-small cell lung cancer, and hepatocellular carcinoma experience abnormally high levels of ATP citrate-lyase.

References

1. Migita T. et al., Cancer Res. 68 (20), 8547- 8554 (2008).
2. Ramakrishna S. et al., Biochemistry 29 (33), 7617-7624 (1990).

Storage/Stability

>6 months at –80°C

Assay Conditions

A 25 µl ACLY reaction is conducted in a buffer containing 40 mM Tris (pH 8.0), 10 mM MgCl₂, 2 mM DTT, 2 mM potassium citrate, 0.1 mM CoA and 6-100 ng human ACLY at 30 oC for 60 min. Enzyme activity was assayed using ADP-Glo reagents (Promega)

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Lyase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

50255 Lot 161107G3.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

ATP Citrate Lyase, GST-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY