ARE Reporter Kit (Nrf2 Antioxidant Pathway )

Catalog #

60514

$650 *

Size: 500 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, which is an Nrf2 pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ARE responsive elements located upstream of a minimal promoter. The ARE reporter is premixed with a constitutively expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. This negative control is critical to determining pathway-specific effects and background luciferase activity.

For assays in more difficult to transfect cell types, we suggest using our lentiviral products. The corresponding lentiviral product for this kit is BPS Bioscience #79869.

This product has been cited 38 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

ARE reporter, Nrf2 reporter, Nrf, Cell based assay kit, cell-based assay, reporter assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations38

Supplied As

Reporter: ARE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Format

Assay Kit

Concentration

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

UniProt #

Q16236

Background

The Nrf2 antioxidant response pathway plays an important role in the cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress and other ARE activators, Nrf2 is released from Keap1 and translocates to nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage.

Storage/Stability

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Instructions for Use

See data sheet insert for detailed transfection and assay protocols.

Applications

• Monitor Nrf2 antioxidant response pathway activity.
• Screen for activators or inhibitors of the Nrf2 antioxidant response pathway.
• Study effects of RNAi or gene overexpression on the activity of the Nrf2 pathway.

Shipping Temperature

-20°C

Notes

The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

Regulatory Status

Research Use Only

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ARE Reporter Kit (Nrf2 Antioxidant Pathway )

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