Anti-TFR Antibody, Biotin-Labeled

Catalog #

101596

$335 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Recombinant anti-TFR (Transferrin Receptor), which recognizes TFR. The purified antibody was enzymatically biotinylated using Avi-tag^TM technology. This affinity purified antibody has been tested for specific binding to purified human TFR1 (BPS Bioscience #101287) in an ELISA binding assay.

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Synonyms

Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, Transferrin receptor protein 1, serum form (sTfR), CD71, TFRC

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

TFR1:Anti-TFR (Biotin) Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Heavy Chain: 50 kDa; Light Chain: 24 kDa + glycans

Glycosylation

This antibody runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt #

P02786

Storage/Stability

At least 12 months at -80°C

Assay Conditions

An ELISA binding assay was performed with this antibody and its target protein, TFR1 (BPS Bioscience #101287). The target protein was coated onto an assay plate overnight at 2 µg/ml at 4°. The next day, the plate was washed on blocked. Then, serial dilutions of the antibody were added (0 to 200 nM) for 1 hour at room temperature. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Then we measured absorbance on a plate reader at 450 nm. EC50 = 0.61 nM

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying the binding of TFR in ELISA and in cellular assays.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy, Neuroscience

Regulatory Status

Research Use Only