Affinity purified Anti-Red Fluorescent Protein (RFP), clone RF5R, Mouse IgG1. Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc. Purified from mouse ascites fluid. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user. Precited M.W. of RFP by Western blot is ~26 kDa.
Synthetic peptide conjugated to KLH, from the N terminal region of Sea anenome (Discosoma) RFP.
Sensitivity
Recognizes 1-10ng of purified RFP or RFP fusion proteins by ECL.
Background
Red fluorescent protein cloned from Discosoma coral (DsRed or drFP583) is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
Storage/Stability
Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.