Anti–Set9 polyclonal antibody

Catalog #

25312

$335 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human Set9 (SET domain containing (lysine methyltransferase) 9), using a recombinant protein.

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Synonyms

SET7, SET7/9, KMT7, H3–K4–HMTase

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Affinity purified

Format

Aqueous buffer solution

Formulation

PBS including 0.05% azide and 0.05% ProClin 300

Concentration

50 µg/25 µl

Immunogen

recombinant protein

Background

Set9 (UniProtKB/Swiss–Prot entry Q8WTS6) is a histone methyltransferase which specifically methylates lysine 4 of histone H3 being a specific tag for epigenetic transcriptional activation. Further, methylation of histone H3K4 by Set9 precludes deacetylation of H3 by the NuRD complex and impairs Suv39h1–mediated methylation of H3K9. Set9 also methylates the tumor suppressor p53 at lysine 372, thereby enhancing its stability. It contains a SET domain but lacks the pre– and post–SET domains.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Western blot analysis using the antibody directed against Set9
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 μg) using the antibody against Set9 (cat. # 25312) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

Applications

WB (1:1000)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–Set9 polyclonal antibody

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