Anti–PHC2 polyclonal antibody

Catalog #

25307

$335 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against mouse PHC2 (polyhomeotic homolog 2) using two KLH–conjugated synthetic peptides containing a sequence from the central region of the protein.

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Synonyms

EDR2, HPH2, PH2

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

PHC2 (UniProtKB/Swiss–Prot entry Q8IXK0) is a component of a Polycomb group (PcG) multiprotein PRC1–like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of lysine 119 on histone H2A, rendering chromatin heritably changed in its expressibility.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against mouse PHC2 (Cat. # 25307). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:33,300.

Applications

ELISA (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Mouse

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–PHC2 polyclonal antibody

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