Anti–H2AK119ub polyclonal antibody

Catalog #

25229

$415 *

Size: 50 µg

Qty

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Description

Polyclonal antibody raised in rabbit against the region of histone H2A containing the ubiquitinylated lysine 119 (H2AK119ub), using a KLH–conjugated synthetic peptide

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Product Data Gallery

ChIP results obtained with the antibody directed against H2AK119ub

Determination of the antibody titer.

Cross reactivity tests using the antibody directed against H2AK119ub

Western blot analysis using the antibody directed against H2AK119ub

Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Affinity purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300

Concentration

1.1 mg/ml

Immunogen

synthetic peptide

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

ChIP results obtained with the antibody directed against H2AK119ub
ChIP assays were performed using human HeLa cells, the antibody against H2AK119ub (cat. No. 25229) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells on the SX-8G IP-Star automated system. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and c-fos genes, used as negative controls, and for the inactive MYT1 and TSH2B genes, used as a positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2AK119ub (cat. No. 25229). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:23,000.

Cross reactivity tests using the antibody directed against H2AK119ub
To test the cross reactivity of the antibody against H2AK119ub (cat. No. 25229), a Dot Blot analysis was performed with peptides containing other histone ubiquitinylations and unmodified sequences. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the modification of interest.

Western blot analysis using the antibody directed against H2AK119ub
Western blot was performed on whole cell extracts from HeLa cells (25 μg, lane 1), and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H2AK119ub (cat. No. 25229). The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

Applications

ChIP (2 – 5 µg/IP)
ELISA (1:200)
DB (1:20,000)
WB (1:200)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Datasheets/Protocols

25229 Lot 230210

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Anti–H2AK119ub polyclonal antibody

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