Anti–ER alpha monoclonal antibody

Catalog #

25221

$415 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Monoclonal antibody raised in mouse against human ERalpha (estrogen receptor alpha), using a synthetic peptide

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Synonyms

ESR, ESR1, ESRA, NR3A14

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Product Info

Storage and Usage

Citations

Clonality

monoclonal

Host Species/Expression System

Mouse

Purification

Ascites fluid

Format

aqueous solution

Formulation

Ascites fluid from mouse containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

The estrogen receptor alpha (ERalpha, UniProt/Swiss–Prot entry P03372) belongs to the family of nuclear hormone receptors, which are ligand–activated transcription factors. They are important for the regulation of gene expression, cellular proliferation and differentiation, sexual development and reproductive function. Estrogen receptors are also involved in pathological processes such as breast cancer, and osteoporosis. ERalpha can regulate transcription by direct binding to estrogen response elements (EREs) in the DNA or by interaction with other transcription factors. It may also form a heterodimer with ERbeta.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

ChIP-seq results obtained with the monoclonal antibody directed against ERalpha
ChIP was performed with the monoclonal antibody against ERalpha (Cat. # 25221) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The immunoprecipitated DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).

Applications

ChIP/ChIP – seq (2.5 µl)
ELISA (1:500 – 1:5000)
WB (1:500 – 1:5000)
Gel Supershift (1:10 – 1:20)
Immunochemistry (1:500 – 1:5000)
IP (1:200 – 1:5000 )

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Cross Reactivity

Does not react with chicken; other species not tested

Notes

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–ER alpha monoclonal antibody

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